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ΔημοσίευσεΚέφαλος Βικελίδης Τροποποιήθηκε πριν 8 χρόνια
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Anti-inflammatory activity of crude extracts from some traditional medicinal plants
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Test Samples (12 종 ) 금은화 ( 金銀花 ) Lonicera japonica (Caprifoliaceae) 두충 ( 杜食 ) Eucommia ulmoides (Eucommiaceae) 만형자 ( 蔓荊子 ) Vitex trifolia (Verbenaceae) 산수유 ( 山茱萸 ) Cornus officinalis (Cornaceae) 생지황 ( 生地黃 ) Rehmanniae glutinosa var. purpurea (Scrophulariaceae) 용담 ( 龍膽 ) Gentiana scabra (Gentianaceae) 인동 ( 忍冬 ) Lonicera japonica (Caprifoliaceae) 정향 ( 丁香 ) Eugenia caryophyllata (Myrtaceae) 차전자 ( 車前子 ) Plantago asiatica (Plantaginaceae) 치자 ( 梔子 ) Gardenia jasminoides (Rubiaceae) 패장 ( 敗醬 ) Patrinia villosa (Valerianaceae) 현삼 ( 玄蔘 ) Scrophularia ningpoensis (Scrophulariae)
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Photos 금은화 두충만형자산수유 생지황용담 인동 정향 차전자치자 패장 현삼
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Objective 약용식물 12 종의 항염작용 고찰 Cytotoxicity (MTT) COX-1 activity (TXB2) COX-2 activity (PEG2) TNFα formation NO production
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Crude Extraction H2OH2O MeOH
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Cytotoxicity (MTT) assay for extracts Principle MTT Formazan reduction by mitochondrial respiration Method RAW 264.7 cell culture and seeding in a 96-well plate LPS and a crude extract (100μg/ml) treatment and incubation for 20hrs at 37 ℃ Incubation with MTT(0.1mg/ml) for 4hrs at 37 ℃ Adding DMSO (200μl) to dissolve formazan crystal Absorbance measurement at 450 and 650nm using automatic ELISA microplate reader
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Cytotoxicity (MTT) assay for extracts Result H2OH2O MeOH LPS(+) 100 μg/ml
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Cytotoxicity (MTT) assay for extracts Result H2OH2O MeOH LPS(+) 100 μg/ml
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Effects of extracts on TXB 2 production HEL cell culture and seeding in a 96-well plate A crude extract (1,10, 50, 100 ㎍ /ml) treatment and incubation for 1hr Arachidonic acid (30 μM ) treatment for 15min Preparation of cell lysate ELISA using anti-TXB 2 monoclonal antibodies IC 50 calculation Method
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Effects of 금은화 Ex on TXB 2 production Result ELISA (n=3) Piroxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 금은화 Ex : 1/10/50/100 μg/ml
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Effects of 두충 Ex on TXB 2 production Result ELISA (n=3) Piroxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 두충 Ex : 1/10/50/100 μg/ml
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Effects of 만형자 Ex on TXB 2 production Result ELISA (n=3) Piroxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 만형자 Ex : 1/10/50/100 μg/ml
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Effects of 산수유 Ex on TXB 2 production Result ELISA (n=3) Piroxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 산수유 Ex : 1/10/50/100 μg/ml
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Effects of 생지황 Ex on TXB 2 production Result ELISA (n=3) Piroxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 생지황 Ex : 1/10/50/100 μg/ml
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Effects of 용담 Ex on TXB 2 production Result ELISA (n=3) Piroxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 용담 Ex : 1/10/50/100 μg/ml
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Effects of 인동 Ex on TXB 2 production Result ELISA (n=3) Piroxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 인동 Ex : 1/10/50/100 μg/ml
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Effects of 정향 Ex on TXB 2 production Result ELISA (n=3) Piroxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 정향 Ex : 1/10/50/100 μg/ml
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Effects of 차전자 Ex on TXB 2 production Result ELISA (n=3) Piroxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 차전자 Ex : 1/10/50/100 μg/ml
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Effects of 치자 Ex on TXB 2 production Result ELISA (n=3) Piroxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 치자 Ex : 1/10/50/100 μg/ml
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Effects of 패장 Ex on TXB 2 production Result ELISA (n=3) Piroxicam : 0.01, 0.1, 1, 10 μM Aspirin : 1, 10, 50, 100 μM 패장 Ex : 1, 10, 50, 100 μg/ml
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Effects of 현삼 Ex on TXB 2 production Result ELISA (n=3) Piroxicam : 0.01, 0.1, 1, 10 μM Aspirin : 1, 10, 50, 100 μM 현삼 Ex : 1, 10, 50, 100 μg/ml
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Effects of extracts on PEG 2 production RAW 264.7 cell culture and seeding in a 96-well plate A crude extract (1,10, 50, 100 ㎍ /ml) treatment and incubation for 1hr Arachidonic acid (30 μM ) treatment for 15min Stimulation with LPS(1 ㎍ /ml) and IFN-γ(10U/ml) for 4hr Preparation of cell lysate ELISA using anti-PGE 2 monoclonal antibodies IC 50 calculation Method
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Effects of 금은화 Ex on PEG 2 production Result ELISA (n=3) Meloxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 금은화 Ex : 1/10/50/100 μg/ml
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Effects of 두충 Ex on PEG 2 production Result ELISA (n=3) Meloxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 두충 Ex : 1/10/50/100 μg/ml * * *
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Effects of 만형자 Ex on PEG 2 production Result ELISA (n=3) Meloxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 만형자 Ex : 1/10/50/100 μg/ml
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Effects of 산수유 Ex on PEG 2 production Result ELISA (n=3) Meloxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 산수유 Ex : 1/10/50/100 μg/ml
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Effects of 생지황 Ex on PEG 2 production Result ELISA (n=3) Meloxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 생지황 Ex : 1/10/50/100 μg/ml
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Effects of 용담 Ex on PEG 2 production Result ELISA (n=3) Meloxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 용담 Ex : 1/10/50/100 μg/ml
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Effects of 인동 Ex on PEG 2 production Result ELISA (n=3) Meloxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 인동 Ex : 1/10/50/100 μg/ml
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Effects of 정향 Ex on PEG 2 production Result ELISA (n=3) Meloxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 정향 Ex : 1/10/50/100 μg/ml
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Effects of 차전자 Ex on PEG 2 production Result ELISA (n=3) Meloxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 차전자 Ex : 1/10/50/100 μg/ml * * *
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Effects of 치자 Ex on PEG 2 production Result ELISA (n=3) Meloxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 치자 Ex : 1/10/50/100 μg/ml
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Effects of 패장 Ex on PEG 2 production Result ELISA (n=3) Meloxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 패장 Ex : 1/10/50/100 μg/ml
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Effects of 현삼 Ex on PEG 2 production Result ELISA (n=3) Meloxicam : 0.01/0.1/1/10 μM Aspirin : 1/10/50/100 μM 현삼 Ex : 1/10/50/100 μg/ml
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Effects of extracts on TNF production RAW 264.7 cell culture and seeding in a 96-well plate A crude extract (1,10, 50, 100 ㎍ /ml) treatment and incubation for 1hr Stimulation with LPS(1 ㎍ /ml) and IFN-γ(10U/ml) for 4hr Preparation of cell lysate ELISA using anti-TNF monoclonal antibodies IC 50 calculation Method
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Effects of 금은화 Ex on TNFα production Result ELISA (n=3) Rolipram : 0.01/0.1/1/10 μM 금은화 Ex : 1/10/50/100 μg/ml
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Effects of 두충 Ex on TNFα production Result ELISA (n=3) Rolipram : 0.01/0.1/1/10 μM 두충 Ex : 1/10/50/100 μg/ml
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Effects of 만형자 Ex on TNFα production Result ELISA (n=3) Rolipram : 0.01/0.1/1/10 μM 만형자 Ex : 1/10/50/100 μg/ml
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Effects of 산수유 Ex on TNFα production Result ELISA (n=3) Rolipram : 0.01/0.1/1/10 μM 산수유 Ex : 1/10/50/100 μg/ml
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Effects of 생지황 Ex on TNFα production Result ELISA (n=3) Rolipram : 0.01/0.1/1/10 μM 생지황 Ex : 1/10/50/100 μg/ml
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Effects of 용담 Ex on TNFα production Result ELISA (n=3) Rolipram : 0.01/0.1/1/10 μM 용담 Ex : 1/10/50/100 μg/ml
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Effects of 인동 Ex on TNFα production Result ELISA (n=3) Rolipram : 0.01/0.1/1/10 μM 인동 Ex : 1/10/50/100 μg/ml
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Effects of 정향 Ex on TNFα production Result ELISA (n=3) Rolipram : 0.01/0.1/1/10 μM 정향 Ex : 1/10/50/100 μg/ml
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Effects of 차전자 Ex on TNFα production Result ELISA (n=3) Rolipram : 0.01/0.1/1/10 μM 차전자 Ex : 1/10/50/100 μg/ml * * *
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Effects of 치자 Ex on TNFα production Result ELISA (n=3) Rolipram : 0.01/0.1/1/10 μM 치자 Ex : 1/10/50/100 μg/ml
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Effects of 패장 Ex on TNFα production Result ELISA (n=3) Rolipram : 0.01/0.1/1/10 μM 패장 Ex : 1/10/50/100 μg/ml
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Effects of 현삼 Ex on TNFα production Result ELISA (n=3) Rolipram : 0.01/0.1/1/10 μM 현삼 Ex : 1/10/50/100 μg/ml
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Effects of extracts on NO production RAW 264.7 cell culture and seeding in a 96-well plate A crude extract (1,10, 50, 100 ㎍ /ml) treatment and incubation for 1hr Stimulation with LPS(1 ㎍ /ml) and IFN-γ(10U/ml) for 24hr Preparation of cell lysate The isolated supernatants were mixed with an equal volume of Griess reagent Nitrite production was determined by measuring the absorbance at 550 nm IC 50 calculation Method
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Effects of 금은화 Ex on NO production Result ELISA (n=3) L -NAME : 1/10/50/100 μM 금은화 Ex : 1/10/50/100 μg/ml
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Effects of 두충 Ex on NO production Result ELISA (n=3) L -NAME : 1/10/50/100 μM 두충 Ex : 1/10/50/100 μg/ml
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Effects of 만형자 Ex on NO production Result ELISA (n=3) L -NAME : 1/10/50/100 μM 만형자 Ex : 1/10/50/100 μg/ml
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Effects of 산수유 Ex on NO production Result ELISA (n=3) L -NAME : 1/10/50/100 μM 산수유 Ex : 1/10/50/100 μg/ml
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Effects of 생지황 Ex on NO production Result ELISA (n=3) L -NAME : 1/10/50/100 μM 생지황 Ex : 1/10/50/100 μg/ml
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Effects of 용담 Ex on NO production Result ELISA (n=3) L -NAME : 1/10/50/100 μM 용담 Ex : 1/10/50/100 μg/ml
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Effects of 정향 Ex on NO production Result ELISA (n=3) L -NAME : 1/10/50/100 μM 정향 Ex : 1/10/50/100 μg/ml
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Effects of 차전자 Ex on NO production Result ELISA (n=3) L -NAME : 1/10/50/100 μM 차전자 Ex : 1/10/50/100 μg/ml * *
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Effects of 치자 Ex on NO production Result ELISA (n=3) L -NAME : 1/10/50/100 μM 치자 Ex : 1/10/50/100 μg/ml
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Effects of 패장 Ex on NO production Result ELISA (n=3) L -NAME : 1/10/50/100 μM 패장 Ex : 1/10/50/100 μg/ml
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Effects of 현삼 Ex on NO production Result ELISA (n=3) L -NAME : 1/10/50/100 μM 현삼 Ex : 1/10/50/100 μg/ml
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IC 50 of aqueous extracts for COX-1, COX-2, TNFα and NO Result COX-1COX-2TNFαNO A- 차전자 -9.99.67.3 A- 두충 -8.6-- IC 50 ( μg/ml )
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